Faculty Information |
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Article types | Original article |
Language | English |
Refereed paper | Refereed |
Title | Celastrol enhances TRAIL‑R2‑mediated apoptosis and cytotoxicity in human renal cell carcinoma cells in caspase‑dependent manner. |
Journal | Formal name:Oncology reports ISSN code:17912431/1021335X |
Domestic / Foregin | Foregin |
Volume, Number, Page | 51(1),pp.12 |
Papers・Author | Bao Yuhang, Wu Xiuxian, Kanematsu Akihiro, Kita Yuki, Kobayashi Takashi, Kakehi Yoshiyuki, Yamamoto Shingo |
Publication date | 2024/01 |
Papers・Description | Celastrol is a triterpene phytochemical known to possess anti‑inflammatory, antioxidant and anticancer properties. The present study investigated the effects of celastrol on tumor necrosis factor‑related apoptosis‑inducing ligand receptor 2 (TRAIL‑R2)‑mediated apoptosis and cytotoxicity in human renal cell carcinoma (RCC) cells when administered in combination with lexatumumab, a human monoclonal agonistic antibody that specifically recognizes TRAIL‑R2. Cytotoxicity was determined by colorimetric assays of cell viability using cell counting kit‑8. The activation of caspases was assessed by quantitative colorimetric assays using caspase‑specific kits. Celastrol significantly enhanced lexatumumab‑induced apoptosis and cytotoxicity in RCC cells. An enhanced effect was also achieved when the duration of treatment with lexatumumab and celastrol was reduced from 24 to 6 h. ... |