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Article types	Original article
Language	English
Refereed paper	Not refereed
Title	Construction of HGF-Displaying Yeast by Cell Surface Engineering.
Journal	Formal name：Microorganisms ISSN code：20762607
Domestic / Foregin	Foregin
Volume, Number, Page	10
Papers・Author	Shibasaki Seiji, Nakatani Yuki, Taketani Kazuaki, Karasaki Miki, Matsui Kiyoshi, Ueda Mitsuyoshi, Iwasaki Tsuyoshi.
Publication date	2022/07
Papers・Description	Hepatocyte growth factor (HGF) has been investigated as a regulator for immune reactions caused by transplantation and autoimmune diseases and other biological functions. Previous studies demonstrated that cDNA-encoding HGF administration could inhibit acute graft-versus-host disease (GVHD) after treatment via hematopoietic stem cell transplantation. This study aimed to show the preparation of HGF protein on yeast cell surfaces to develop a tool for the oral administration of HGF to a GVHD mouse model. In this study, full-length HGF and the heavy chain of HGF were genetically fused with α-agglutinin and were successfully displayed on the yeast cell surface. This study suggested that yeast cell surface display engineering could provide a novel administration route for HGF.