教員業績データベース |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読なし |
表題 | Construction of HGF-Displaying Yeast by Cell Surface Engineering. |
掲載誌名 | 正式名:Microorganisms ISSNコード:20762607 |
掲載区分 | 国外 |
巻・号・頁 | 10 |
著者・共著者 | Shibasaki Seiji, Nakatani Yuki, Taketani Kazuaki, Karasaki Miki, Matsui Kiyoshi, Ueda Mitsuyoshi, Iwasaki Tsuyoshi. |
発行年月 | 2022/07 |
概要 | Hepatocyte growth factor (HGF) has been investigated as a regulator for immune reactions caused by transplantation and autoimmune diseases and other biological functions. Previous studies demonstrated that cDNA-encoding HGF administration could inhibit acute graft-versus-host disease (GVHD) after treatment via hematopoietic stem cell transplantation. This study aimed to show the preparation of HGF protein on yeast cell surfaces to develop a tool for the oral administration of HGF to a GVHD mouse model. In this study, full-length HGF and the heavy chain of HGF were genetically fused with α-agglutinin and were successfully displayed on the yeast cell surface. This study suggested that yeast cell surface display engineering could provide a novel administration route for HGF. |