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Article types	Original article
Language	English
Refereed paper	Refereed
Title	Risk prediction for metastasis of clear cell renal cell carcinoma using digital multiplex ligation-dependent probe amplification.
Journal	Formal name：Cancer science ISSN code：13497006/13479032
Domestic / Foregin	Domestic
Volume, Number, Page	113(1),pp.297-307
Papers・Author	Yoshikawa Yoshie, Yamada Yusuke, Emi Mitusru, Lilit Atanesyan, Jan Smout, Karel de Groot, Suvi Savola, Nakanishi-Shinkai Yukako, Kanematsu Akihiro, Nojima Mmichio, Ohmuraya Masaki, Hashimoto-Tamaoki Tomoko, Yamamoto Shingo
Publication date	2022/01
Papers・Description	Precise quantification of copy-number alterations (CNAs) in a tumor genome is difficult. We have applied a comprehensive copy-number analysis method, digital multiplex ligation-dependent probe amplification (digitalMLPA), for targeted gene copy-number analysis in clear cell renal cell carcinoma (ccRCC). Copy-number status of all chromosomal arms and 11 genes was determined in 60 ccRCC samples. Chromosome 3p loss and 5q gain, known as early changes in ccRCC development, as well as losses at 9p and 14q were detected in 56/60 (93.3%), 31/60 (51.7%), 11/60 (18.3%), and 33/60 (55%), respectively.
PMID	34687579