Faculty Information |
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Article types | Original article |
Language | English |
Refereed paper | Refereed |
Title | Circadian coordination of ATP release in the urothelium via connexin43 hemichannels. |
Journal | Formal name:Scientific reports Abbreviation:Sci Rep ISSN code:2045-2322(Electronic)2045-2322(Linking) |
Domestic / Foregin | Foregin |
Volume, Number, Page | 8(1),pp.1996 |
Papers・Author | Sengiku Atsushi, Ueda Masakatsu, Kono Jin, Sano Takeshi, Nishikawa Nobuyuki, Kunisue Sumihiro, Tsujihana Kojiro, Liou Louis S, Kanematsu Akihiro, Shimba Shigeki, Doi Masao, Okamura Hitoshi, Ogawa Osamu, Negoro Hiromitsu |
Publication date | 2018/01 |
Papers・Description | Day-night changesin the storage capacity of the urinary bladder are indispensable for sound sleep. Connexin 43 (Cx43), a major gap junction protein, forms hemichannels as a pathway of ATP in other cell types, and the urinary bladder utilizes ATP as a mechanotransduction signals to modulate its capacity. Here, we demonstrate that the circadian clock of the urothelium regulates diurnal ATP release through Cx43 hemichannels. Cx43 was expressed in human and mouse urothelium, and clock genes oscillated in the mouse urothelium accompanied by daily cycles in the expression of Cx43 and extracellular ATP release into the bladder lumen. Equivalent chronological changes in Cx43 and ATP were observed in immortalized human urothelial cells, but these diurnal changes were lost in both arrhythmic Bmal1-knockout mice and in BMAL1-knockdown urothelial cells. |
DOI | 10.1038/s41598-018-20379-0 |
PMID | 29386573 |