教員業績データベース |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | Circadian coordination of ATP release in the urothelium via connexin43 hemichannels. |
掲載誌名 | 正式名:Scientific reports 略 称:Sci Rep ISSNコード:2045-2322(Electronic)2045-2322(Linking) |
掲載区分 | 国外 |
巻・号・頁 | 8(1),pp.1996 |
著者・共著者 | Sengiku Atsushi, Ueda Masakatsu, Kono Jin, Sano Takeshi, Nishikawa Nobuyuki, Kunisue Sumihiro, Tsujihana Kojiro, Liou Louis S, Kanematsu Akihiro, Shimba Shigeki, Doi Masao, Okamura Hitoshi, Ogawa Osamu, Negoro Hiromitsu |
発行年月 | 2018/01 |
概要 | Day-night changesin the storage capacity of the urinary bladder are indispensable for sound sleep. Connexin 43 (Cx43), a major gap junction protein, forms hemichannels as a pathway of ATP in other cell types, and the urinary bladder utilizes ATP as a mechanotransduction signals to modulate its capacity. Here, we demonstrate that the circadian clock of the urothelium regulates diurnal ATP release through Cx43 hemichannels. Cx43 was expressed in human and mouse urothelium, and clock genes oscillated in the mouse urothelium accompanied by daily cycles in the expression of Cx43 and extracellular ATP release into the bladder lumen. Equivalent chronological changes in Cx43 and ATP were observed in immortalized human urothelial cells, but these diurnal changes were lost in both arrhythmic Bmal1-knockout mice and in BMAL1-knockdown urothelial cells. |
DOI | 10.1038/s41598-018-20379-0 |
PMID | 29386573 |