Faculty Information |
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Article types | Original article |
Language | English |
Refereed paper | Not refereed |
Title | Immunohistochemical characterization of cancer-associated fibroblasts at the primary sites and in the metastatic lymph nodes of human intrahepatic cholangiocarcinoma |
Journal | Formal name:Human pathology Abbreviation:Hum Pathol ISSN code:1532-8392(Electronic)0046-8177(Linking) |
Domestic / Foregin | Foregin |
Volume, Number, Page | 83,pp.77-89 |
Papers・Author | Itou Rei Atono, Uyama Naoki, Hirota Seiichi, Kawada Norifumi, Wu Songtao, Miyashita Seikan, Nakamura Ikuo, Suzumura Kazuhiro, Sueoka Hideaki, Okada Tosihiro, Hatano Etsuro, Tsutsui Hiroko, Fujimoto Jiro |
Publication date | 2018/08 |
Papers・Description | Cancer-associated fibroblasts (CAFs) are an important constituent of the cancer stroma. In intrahepatic cholangiocarcinoma (ICC), the features of CAFs at the primary site and in the metastatic lymph nodes (Met-LNs) and their origin have been unclear. In the present study, we characterized CAFs at the primary site (n=42) and in the Met-LNs (n=10) of human ICC by immunohistochemistry using potential molecular markers of CAFs, portal fibroblasts (PFs), hepatic stellate cells (HSCs) and bone marrow-derived fibrocytes (BMDFs). At the primary site, the stroma was strongly positive for α-SMA (marker for CAFs), PDGFR-β (common marker for HSCs and PFs), fibulin-2 and Thy-1 (PF marker), while immunoreactivity for fascin (HSC marker) was scarce. A majority of the α-SMA-positive cells were found to express PDGFR-β, Thy-1 and fibulin-2 by double immunostaining. A small population of BMDF marker-positive (α-SMA+ |
DOI | 10.1016/j.humpath.2018.08.016 |
PMID | 30172911 |