Faculty Information |
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Article types | Original article |
Language | English |
Refereed paper | Refereed |
Title | The cytokine polymorphisms affecting Th1/Th2 increase the susceptibility to, and severity of, chronic ITP. |
Journal | Formal name:BMC immunology Abbreviation:BMC Immunol ISSN code:1471-2172(Electronic)1471-2172(Linking) |
Domestic / Foregin | Foregin |
Volume, Number, Page | 18(1),pp.26 |
Papers・Author | Takahashi Noriyuki, Saitoh Takayuki, Gotoh Nanami, Nitta Yasuhiro, Alkebsi Lobna, Kasamatsu Tetsuhiro, Minato Yusuke, Yokohama Akihiko, Tsukamoto Norifumi, Handa Hiroshi, Murakami Hirokazu |
Publication date | 2017/05 |
Papers・Description | BACKGROUND:T-helper cell type 1 (Th1) polarization in chronic immune thrombocytopenia (cITP) has been reported at the protein and mRNA levels. We evaluated the impact of Th1/Th2 cytokine and cytokine receptor functional polymorphisms on both susceptibility to, and severity of, cITP. We analysed IFN-γ + 874 T/A, IFN-γR -611G/A, IL-4 -590C/T, and IL-4Rα Q576R polymorphisms in 126 cITP patients (male/female: 34/92; median age: 47.7 years) and 202 healthy control donors. Genotyping was determined by PCR and direct sequencing. The Th1/Th2 ratio was detected in peripheral blood mononuclear cells via flow cytometry.RESULTS:cITP patients had a higher frequency of the IL-4Rα 576 non-QQ genotype compared to healthy subjects (P = 0.04). cITP patients with the IFN-γ +874 non-AA genotype (high expression type) showed more severe thrombocytopenia than those with the AA genotype (P < 0.05). cITP patients had a significantly higher Th1/Th2 ratio than control patients (P < 0.01); this ratio was inversely correlated with platelet counts. Furthermore, patients with both IFN-γ +874 non-AA genotype (high expression type) and IFN-γR -611 non-AA genotype (high-function type) had a significantly higher Th1/Th2 ratio (P < 0.05).CONCLUSIONS:The cytokine polymorphisms affecting Th1/Th2 increase the susceptibility to, and severity of, chronicITP. |
DOI | 10.1186/s12865-017-0210-3 |
PMID | 28511637 |