Faculty Information |
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Article types | Original article |
Language | English |
Refereed paper | Refereed |
Title | Specific circulating microRNAs display dose-dependent responses to variable intensity and duration of endurance exercise. |
Journal | Formal name:American journal of physiology. Heart and circulatory physiology Abbreviation:Am J Physiol Heart Circ Physiol ISSN code:1522-1539(Electronic)0363-6135(Linking) |
Domestic / Foregin | Foregin |
Volume, Number, Page | 315(2),pp.H273-83 |
Papers・Author | Ramos Anna E, Lo Claire, Estephan Leonard E, Tai Yi-Yin, Tang Ying, Zhao Jingsi, Sugahara Masataka, Gorcsan John, Brown Marcel G, Lieberman Daniel E, Chan Stephen Y, Baggish Aaron L |
Publication date | 2018/08 |
Papers・Description | Circulating microRNAs (c-miRNAs), plasma-based noncoding RNAs that control posttranscriptional gene expression, mediate processes that underlie phenotypical plasticity to exercise. The relationship and biological relevance between c-miRNA expression and variable dose exercise exposure remains uncertain. We hypothesized that certain c-miRNAs respond to changes in exercise intensity and/or duration in a dose-dependent fashion. Muscle release of such c-miRNAs may then deplete intracellular stores, thus facilitating gene reprogramming and exercise adaptation. To address these hypotheses, healthy men participated in variable intensity ( n = 12, 30 × 1 min at 6, 7, and 8 miles/h, order randomized) and variable duration ( n = 14, 7 × 1 mile/h for 30, 60, and 90 min, order randomized) treadmill-running protocols. Muscle-enriched c-miRNAs (i.e., miRNA-1 and miRNA-133a) and others with known relevance to exercise were measured before and after exercise. c-miRNA responses followed three profiles: 1) nonresponsive (miRNA-21 and miRNA-210), 2) responsive to exercise at some threshold but without dose dependence (miRNA-24 and miRNA-146a), and 3) responsive to exercise with dose dependence to increasing intensity (miRNA-1) or duration (miRNA-133a and miRNA-222). We also studied aerobic exercise-trained mice, comparing control, low-intensity (0.5 km/h), or high-intensity (1 km/h) treadmill-running protocols over 4 wk. In high- but not low-intensity-trained mice, we found increased plasma c-miR-133a along with decreased intracellular miRNA-133a and increased serum response factor, a known miR-133a target gene, in muscle. Characterization of c-miRNAs that are dose responsive to exercise in humans and mice supports the notion that they directly mediate physiological adaptation to exercise, potentially through depletion of intracellular stores of muscle-specific miRNAs. NEW&NOTEWORTHY In this study of humans and mice, we define circulating microRNAs in plasma that are dose responsive t |
DOI | 10.1152/ajpheart.00741.2017 |
PMID | 29600898 |