Faculty Information |
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Article types | Original article |
Language | English |
Refereed paper | Refereed |
Title | Inhibition of Asparagine-linked Glycosylation Participates in Hypoxia-induced Down-regulation of Cell-surface MICA Expression. |
Journal | Formal name:Anticancer research Abbreviation:Anticancer Res ISSN code:1791-7530(Electronic)0250-7005(Linking) |
Domestic / Foregin | Foregin |
Volume, Number, Page | 38(3),pp.1353-9 |
Papers・Author | Yamada Naoko, Kato-Kogoe Nahoko, Yamanegi Koji, Nishiura Hiroshi, Fujihara Yuki, Fukunishi Shigeo, Okamura Haruki, Terada Nobuyuki, Nakasho Keiji |
Publication date | 2018/03 |
Papers・Description | BACKGROUND/AIM:Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding. Recently, the inhibition of N-linked glycosylation was also shown to reduce the cell-surface expression of MICA. We investigated the participation of asparagine (Asn)-linked glycosylation in hypoxia-induced down-regulation of cell-surface MICA using osteosarcoma cells.MATERIALS AND METHODS:The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively.RESULTS:Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA. 2-Deoxy-D-glucose, which inhibits N-linked glycosylation, reduced the cell-surface expression of MICA under normoxia, while D-Mannose increased N-glycosylated MICA, increasing cell-surface MICA under hypoxia. Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation.CONCLUSION:The inhibition of Asn-N-linked glycosylation participates in hypoxia-induced down-regulation of cell-surface expression of MICA. |
DOI | 10.21873/anticanres.12358 |
PMID | 29491059 |