教員業績データベース |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | The roles of a ribosomal protein S19 polymer in a mouse model of carrageenan-induced acute pleurisy. |
掲載誌名 | 正式名:Immunobiology 略 称:Immunobiology ISSNコード:1878-3279(Electronic)0171-2985(Linking) |
掲載区分 | 国外 |
巻・号・頁 | 222(5),pp.738-50 |
著者・共著者 | Yamanegi Koji, Kawakami Toru, Yamada Naoko, Kumanishi Shunsuke, Futani Hiroyuki, Nakasho Keiji, Nishiura Hiroshi |
発行年月 | 2017/05 |
概要 | C5-deficient mice usually present moderate neutrophil activation during the initiation phase of acute inflammation. Conversely, C5a receptor (C5aR)-deficient mice show unusually excessive activation of neutrophils. We identified the ribosomal protein S19 (RP S19) polymer, which is cross-linked at Lys122 and Gln137 by transglutaminases in apoptotic neutrophils, as a second C5aR ligand during the resolution phase of acute inflammation. The RP S19 polymer promotes apoptosis via the neutrophil C5aRand phagocytosis via the macrophage C5aR. To confirm the roles of the RP S19 polymer, we employed a carrageenan-induced acute pleurisy mouse model using C57BL/6J mice with a knock-in of the Gln137Glu mutant RP S19 gene and replaced the RP S19 polymer with either an S-tagged C5a/RP S19 recombinant protein or the RP S19(122-145) peptide monomer and dimer (as functional C5aR agonists/antagonists) and the RP S19(122-145) peptide trimer (as a functional C5aR antagonist). Neutrophils and macrophages were still present in the thoracic cavities of the knock-in mice at 24h and 7days after carrageenan injection, respectively. Knock-in mice showed structural organization and severe hemorrhaging from the surrounding small vessels of the alveolar walls in the lung parenchyma. In contrast to the RP S19(122-145) peptide monomer and trimer, the simultaneous presence of S-tagged C5a/RP S19 and the RP S19(122-145) peptide dimer completely improved the physiological and pathological acute inflammatory cues. The RP S19 polymer, especially the dimer, appears to play a role at the resolution phase of carrageenan-induced acute pleurisy in C57BL/6J model mice. |
DOI | 10.1016/j.imbio.2017.02.001 |
PMID | 28190533 |