教員業績データベース |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | Human HLA-Ev (147) Expression in Transgenic Animals |
掲載誌名 | 正式名:Transplantation proceedings 略 称:Transplant Proc ISSNコード:1873-2623(Electronic)0041-1345(Linking) |
掲載区分 | 国外 |
巻・号・頁 | 48(4),pp.1323-5 |
著者・共著者 | Matsuura R, Maeda A, Sakai R, Eguchi H, Lo P-C, Hasuwa H, Ikawa M, Nakahata K, Zenitani M, Yamamichi T, Umeda S, Deguchi K, Okuyama H, Miyagawa S |
発行年月 | 2016/05 |
概要 | BACKGROUND:In our previous study, we reported on the development of substituting S147C for HLA-E as a useful gene tool for xenotransplantation. In this study we exchanged the codon of HLA-Ev (147), checked its function, and established a line of transgenic mice.METHODS:A new construct, a codon exchanging human HLA-Ev (147) + IRES + human beta 2-microgloblin, was established. The construct was subcloned into pCXN2 (the chick beta-actin promoter and cytomegalovirus enhancer) vector. Natural killer cell- and macrophage-mediated cytotoxicities were performed using the established the pig endothelial cell (PEC) line with the new gene. Transgenic mice with it were next produced using a micro-injection method.RESULTS:The expression of the molecule on PECs was confirmed by the transfection of the plasmid. The established molecules on PECs functioned well in regulating natural killer cell-mediated cytotoxicity and macrophage-mediated cytotoxicity. We have also successfully generated several lines of transgenic mice with this plasmid. The expression of HLA-Ev (147) in each mouse organ was confirmed by assessing the mRNA. The chick beta-actin promoter and cytomegalovirus enhancer resulted in a relatively broad expression of the gene in each organ, and a strong expression in the cases of the heart and lung.CONCLUSION:A synthetic HLA-Ev (147) gene with a codon usage optimized to a mammalian system represents a critical factor in the development of transgenic animals for xenotransplantation. |
DOI | 10.1016/j.transproceed.2015.10.069 |
PMID | 27320614 |