Faculty Information |
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Article types | Original article |
Language | English |
Refereed paper | Refereed |
Title | Interferon γ-Induced Nuclear Interleukin-33 Potentiates the Release of Esophageal Epithelial Derived Cytokines. |
Journal | Formal name:PloS one Abbreviation:PLoS One ISSN code:1932-6203(Electronic)1932-6203(Linking) |
Domestic / Foregin | Foregin |
Volume, Number, Page | 11(3),pp.e0151701 |
Papers・Author | Shan Jing, Oshima Tadayuki, Wu Liping, Fukui Hirokazu, Watari Jiro, Miwa Hiroto |
Publication date | 2016/03 |
Papers・Description | BACKGROUND:Esophageal epithelial cells are an initiating cell type in esophageal inflammation, playing an essential role in the pathogenesis of gastroesophageal reflux disease (GERD). A new tissue-derived cytokine, interleukin-33 (IL-33), has been shown to be upregulated in esophageal epithelial cell nuclei in GERD, taking part in mucosal inflammation. Here, inflammatory cytokines secreted by esophageal epitheBACKGROUND:lial cells, and their regulation by IL-33, were investigated.METHODS:In an in vitro stratified squamous epithelial model, IL-33 expression was examined using quantitative RT-PCR, western blot, ELISA, and immunofluorescence. Epithelial cell secreted inflammatory cytokines were examined using multiplex flow immunoassay. IL-33 was knocked down with small interfering RNA (siRNA) in normal human esophageal epithelial cells (HEECs). Pharmacological inhibitors and signal transducers and activators of transcription 1 (STAT1) siRNA were used to explore the signaling pathways.RESULTS:Interferon (IFN)γ treatment upregulated nuclear IL-33 in HEECs. Furthermore, HEECs can produce various inflammatory cytokines, such as IL-6, IL-8, monocyte chemoattractant protein 1 (MCP-1), regulated on activation normal T-cell expressed and presumably secreted (RANTES), and granulocyte-macrophage colony-stimulating factor (GM-CSF) in response to IFNγ. Nuclear, but not exogenous IL-33, amplified IFN induction of these cytokines. P38 mitogen-activated protein kinase (MAPK) and janus protein tyrosine kinases (JAK)/STAT1 were the common signaling pathways of IFNγ-mediated induction of IL-33 and other cytokines.CONCLUSIONS:Esophageal epithelial cells can actively participate in GERD pathogenesis through the production of various cytokines, and epithelial-derived IL-33 might play a central role in the production of these cytokines. |
DOI | 10.1371/journal.pone.0151701 |
PMID | 26986625 |