教員業績データベース |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | Esophageal Epithelial-Derived IL-33 Is Upregulated in Patients with Heartburn. |
掲載誌名 | 正式名:PloS one 略 称:PLoS One ISSNコード:1932-6203(Electronic)1932-6203(Linking) |
掲載区分 | 国外 |
巻・号・頁 | 11(4),pp.e0154234 |
著者・共著者 | Sei Hiroo, Oshima Tadayuki, Shan Jing, Wu Liping, Yamasaki Takahisa, Okugawa Takuya, Kondo Takashi, Tomita Toshihiko, Fukui Hirokazu, Watari Jiro, Miwa Hiroto |
発行年月 | 2016/05 |
概要 | BACKGROUND:Interleukin-33 (IL-33) is a tissue-derived cytokine that is constitutively expressed in epithelial cells of tissues exposed to the environment and plays a role in sensing damage caused by inflammatory diseases. IL-33 acts as both a traditional cytokine and as a chromatin-associated nuclear factor in both innate and adaptive immunity. We recently showed that IL-33 in esophageal mucosa is upregulated in reflux esophagitis. However, IL-33 expression in patients with heartburn without mucosal injury and its relationship with intercellular space (ICS) have never been examined. We therefore examined the expression of cytokines and ICS in patients with heartburn.METHODS:The expression of IL-33 in the middle and distal esophageal mucosa of patients with heartburn without mucosal break and control samples was examined using real-time RT-PCR and immunofluorescence. The mRNA expression of IL-6, IL-8, MCP-1, and RANTES, and ICS was also analyzed.RESULTS:IL-33 expression and the mean ICS were significantly increased in the mucosa of patients with heartburn compared to that of the control. IL-33 and ICS were not differenRESULTS:t between the patients who were taking a PPI and those who were not. The upregulated IL-33 expression in the heartburn group was located in the nuclei of the basal cell layer. Although IL-6, IL-8, MCP-1 and RANTES levels were not different between control and patients with heartburn samples, IL-33 mRNA levels were still significantly correlated with IL-6, IL-8, or MCP-1 mRNA levels.CONCLUSION:Nuclear IL-33 is upregulated in patients with heartburn. Upregulated IL-33 in heartburn patients is related to the symptoms. |
DOI | 10.1371/journal.pone.0154234 |
PMID | 27111066 |