教員業績データベース |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読なし |
表題 | Linezolid-resistant Staphylococcus aureus isolated from 2006 through 2008 at six hospitals in Japan. |
掲載誌名 | 正式名:Kansenshoogaku zasshi. The Journal of the Japanese Association for Infectious Diseases 略 称:Kansenshogaku Zasshi ISSNコード:0387-5911(Print)0387-5911(Linking) |
掲載区分 | 国外 |
巻・号・頁 | Suppl 13,pp.8-14 |
著者・共著者 | Ikeda-Dantsuji Yurika, Hanaki Hideaki, Sakai Fuminori, Tomono Kazunori, Takesue Yoshio, Honda Junichi, Nonomiya Yuriko, Suwabe Akira, Nagura Osanori, Yanagihara Katsunori, Mikamo Hiroshige, Fukuchi Kunihiko, Kaku Mitsou, Kohno Shigeru, Yanagisawa Chie, Nakae Taiji, Yoshida Koichiro, Niki Yoshihito |
発行年月 | 2015/07 |
概要 | Limited use of linezolid for treating methicillin-resistant Staphylococcus aureus (MRSA) infection was approved in Japan in 2006. We report here the status of linezolid-resistant MRSAs in Japan. Eleven linezolid-resistant clinical isolates from 11 patients at six hospitals were collected from 2006 through 2008. The minimal inhibitory concentration (MIC) of linezolid in these strains varied from 8 to 64 μg/ml. All strains had at least one G2576T mutation in the chromosomal gene(s) encoding domain V of the 23S ribosomal RNA (rRNA). Chromosomal DNA encoding five copies of the domain V region was analyzed by polymerase chain reaction (PCR). Strains with the linezolid MICs of 64, 32, 16, and 8 μg/ml had the G2576T mutation(s) in four, three (or four), two, and one copy of the 23S rRNA genes, respectively. These results suggest that the level of linezolid resistance seems to be roughly correlated with the number of mutations in the genes encoding 23S rRNA. DNA samples from all 11 strains were subjected to pulsed-field gel electrophoresis and were classified into seven independent clones having>92% identity. Among the 11 patients, five had been treated with linezolid and the remainder, in two hospitals, had no history of prior linezolid use. The results suggested possible nosocomial infections by linezolid-resistant MRSA. |
PMID | 26529982 |