教員業績データベース |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | Varicella zoster virus antibody detection: A comparison of four commonly used techniques. |
掲載誌名 | 正式名:Journal of infection and chemotherapy 略 称:J Infect Chemother ISSNコード:1437-7780(Electronic)21X(Linking) |
巻・号・頁 | 22(4),pp.225-8 |
著者・共著者 | Otani Naruhito, Tanaka Miyuki, Maeda Kazuhiro, Gomi Yasuyuki, Nakajima Kazuhiko, Tanimura Susumu, Takesue Yoshio, Shima Masayuki, Okuno Toshiomi |
発行年月 | 2016/04 |
概要 | BACKGROUND:Antibody tests for the varicella zoster virus (VZV) include neutralization, fluorescent antibody to membrane antigen (FAMA), immune adherence hemagglutination (IAHA), enzyme immunoassay (EIA), glycoprotein-based enzyme-linked immunosorbent assay (gpELISA), and complement fixation (CF) tests. Of these, FAMA is considered the most sensitive. However, in Japan, the EIA method is most frequently employed.OBJECTIVE:The VZV antibody detection rate of the FAMA, EIA, gpELISA, and IAHA methods was compared.METHODS:Four types of antibody tests were conducted with sera collected from 83 college students. The relationships between two antibody tests were examined using Pearson's correlation coefficients.RESULTS:All 83 subjects were observed to be VZV antibody-positive using the FAMA method. The Pearson correlation coefficients of gpELISA, EIA, and IAHA relative to FAMA were 0.808, 0.782, and 0.356, respectively. The positive agreement rate of IAHA relative to FAMA was 88.0% (73/83), whereas those of gpELISA and EIA were both 97.6% (81/83). Furthermore, EIA showed 100% positive agreement with gpELISA and a high correlation coefficient of 0.911, whereas these values for IAHA compared to gpELISA were much lower (90.1% and 0.530). The calculated Pearson correlation coefficient for comparison of the EIA and IAHA methods was 0.498, with a positive agreement rate of 90.1% (73/81).CONCLUSIONS:The EIA method should be employed in Japan based on the similarity of the positivity between EIA and gpELISA, as it is more available and practical than gpELISA. |
DOI | 10.1016/j.jiac.2015.12.018 |
PMID | 26867792 |