Faculty Information |
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Article types | Original article |
Language | English |
Refereed paper | Refereed |
Title | Junctional adhesion molecule-A promotes proliferation and inhibits apoptosis of gastric cancer. |
Journal | Formal name:Hepato-gastroenterology Abbreviation:Hepatogastroenterology ISSN code:0172-6390(Print)0172-6390(Linking) |
Volume, Number, Page | 62(138),pp.540-5 |
Papers・Author | Ikeo Koichi, Oshima Tadayuki, Shan Jing, Matsui Hirofumi, Tomita Toshihiko, Fukui Hirokazu, Watari Jiro, Miwa Hiroto |
Publication date | 2015/07 |
Papers・Description | BACKGROUND/AIMS:Junctional adhesion molecules (JAMs) are known as integral constituents of cellular tight junctions. However, the functions of JAMs in cancer tissues are controversial and the function of JAM-A in gastric cancer is unclear. Acordingly, we investigated the function of JAM-A in gastric epBACKGROUND/AIMS:ithelial and gastric cancer cell proliferation, invasion and apoptosis.METHODOLOGY:A normal rat gastric mucosa-derived cell line (RGM1), a rat gastric cancer-like cell line established from RGM1 (RGK1), and a human gastric cancer cell line (NCI-N87) were used in this study. To examine the expression of junctional proteins, immunoblotting and immunofluorescent staining were performed with specific antibodies (JAM-A, claudins, occludin and ZO-1). JAM-A was knocked down by small interfering RNA.RESULTS:RGM1 and RGK1 expressed JAM-A, occludin and ZO-1 but not claudins. RGK1 were significantly more invasive than RGM1. JAM-A knock-down significantly decreased the proliferation and the invasion of RGK1 but not of RGM1. JAM-A knock-down significantly decreased the proliferation of NCI-N87 cells and significantly decreased expression of the anti-apoptotic protein Bcl-xL but not the expression of AKT or Mcl-1.CONCLUSIONS:JAM-A promotes proliferation and inhibits apoptosis of gastric cancer, suggesting that it has a pivotal role in gastric cancer progression. |
PMID | 25916097 |