教員業績データベース |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | Junctional adhesion molecule-A promotes proliferation and inhibits apoptosis of gastric cancer. |
掲載誌名 | 正式名:Hepato-gastroenterology 略 称:Hepatogastroenterology ISSNコード:0172-6390(Print)0172-6390(Linking) |
巻・号・頁 | 62(138),pp.540-5 |
著者・共著者 | Ikeo Koichi, Oshima Tadayuki, Shan Jing, Matsui Hirofumi, Tomita Toshihiko, Fukui Hirokazu, Watari Jiro, Miwa Hiroto |
発行年月 | 2015/07 |
概要 | BACKGROUND/AIMS:Junctional adhesion molecules (JAMs) are known as integral constituents of cellular tight junctions. However, the functions of JAMs in cancer tissues are controversial and the function of JAM-A in gastric cancer is unclear. Acordingly, we investigated the function of JAM-A in gastric epBACKGROUND/AIMS:ithelial and gastric cancer cell proliferation, invasion and apoptosis.METHODOLOGY:A normal rat gastric mucosa-derived cell line (RGM1), a rat gastric cancer-like cell line established from RGM1 (RGK1), and a human gastric cancer cell line (NCI-N87) were used in this study. To examine the expression of junctional proteins, immunoblotting and immunofluorescent staining were performed with specific antibodies (JAM-A, claudins, occludin and ZO-1). JAM-A was knocked down by small interfering RNA.RESULTS:RGM1 and RGK1 expressed JAM-A, occludin and ZO-1 but not claudins. RGK1 were significantly more invasive than RGM1. JAM-A knock-down significantly decreased the proliferation and the invasion of RGK1 but not of RGM1. JAM-A knock-down significantly decreased the proliferation of NCI-N87 cells and significantly decreased expression of the anti-apoptotic protein Bcl-xL but not the expression of AKT or Mcl-1.CONCLUSIONS:JAM-A promotes proliferation and inhibits apoptosis of gastric cancer, suggesting that it has a pivotal role in gastric cancer progression. |
PMID | 25916097 |