Faculty Information |
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Article types | Original article |
Language | English |
Refereed paper | Refereed |
Title | Association between renal iron accumulation and renal interstitial fibrosis in a rat model of chronic kidney disease. |
Journal | Formal name:Hypertension research : official journal of the Japanese Society of Hypertension Abbreviation:Hypertens Res ISSN code:1348-4214(Electronic)0916-9636(Linking) |
Volume, Number, Page | 38(7),pp.463-70 |
Papers・Author | Naito Yoshiro, Fujii Aya, Sawada Hisashi, Oboshi Makiko, Iwasaku Toshihiro, Okuhara Yoshitaka, Morisawa Daisuke, Eguchi Akiyo, Hirotani Shinichi, Masuyama Tohru |
Publication date | 2015/07 |
Papers・Description | Iron accumulation is associated with the pathophysiology of chronic kidney disease (CKD). Renal fibrosis is a final common feature that contributes to the progression of CKD; however, little is known about the association between renal iron accumulation and renal interstitial fibrosis in CKD. Here we investigate the effects of iron chelation on renal interstitial fibrosis in a rat model of CKD. CKD was induced by 5/6 nephrectomy in Sprague-Dawley rats. At 8 weeks after operation, 5/6 nephrectomized rats were administered an oral iron chelator, deferasirox (DFX), in chow for 8 weeks. Other CKD ratswere given a normal diet. Sham-operative rats given a normal diet served as a control. CKD rats exhibited hypertension, glomerulosclerosis and renal interstitial fibrosis. Iron chelation with DFX did not change hypertension and glomerulosclerosis; however, renal interstitial fibrosis was attenuated in CKD rats. Consistent with these findings, renal gene expression of collagen type III and transforming growth factor-β was increased in CKD rats compared with the controls, while iron chelation suppressed these increments. In addition, a decrease in vimentin along an increase in E-cadherin in renal gene expression was observed in CKD rats with iron chelation. CKD rats also showed increased CD68-positive cells in the kidney, whereas its increase was attenuated by iron deprivation. Similarly, increased renal gene expression of CD68, tumor necrosis factor-α and monocyte chemoattractant protein-1 was suppressed in CKD rats with iron chelation. Renal iron accumulation seems to be associated with renal interstitial fibrosis in a rat model of CKD. |
DOI | 10.1038/hr.2015.14 |
PMID | 25693854 |