教員業績データベース |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | Development of a simplified and convenient assay for cell-mediated immunity to the mumps virus. |
掲載誌名 | 正式名:Journal of immunological methods 略 称:J Immunol Methods ISSNコード:1872-7905(Electronic)0022-1759(Linking) |
巻・号・頁 | 411,pp.50-4 |
著者・共著者 | Otani Naruhito, Shima Masayuki, Nakajima Kazuhiko, Takesue Yoshio, Okuno Toshiomi |
発行年月 | 2014/09 |
概要 | Because methods for measuring cell-mediated immunity (CMI) to the mumps virus are expensive, time-consuming, and technically demanding, the role of CMI in mumps virus infection remains unclear. To address this issue, we report here the development of a simplified method for measuring mumps virus-specific CMI that is suitable for use in diverse laboratory and clinical settings. A mumps vaccine was cultured with whole blood, and interferon (IFN)-γ released into the culture supernatant was measured using an enzyme-linked immunosorbent assay. IFN-γ production in blood from vaccinated subjects markedly increased in response to the vaccine and decreased before the antibody titer decreased in some cases, suggesting that this assay may be used as a simple surrogate method for measuring CMI specific for the mumps virus. |
DOI | 10.1016/j.jim.2014.06.006 |
PMID | 24932836 |